Cellular & Molecular Imaging

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

Asymmetric localization of oskar RNP granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP granules in the oocyte are condensates with solid-like physical properties. Using purified oskar RNA and scaffold proteins Bruno and Hrp48, we confirm in vitro that oskar granules undergo a liquid-to-solid phase transition. Whereas the liquid phase allows RNA incorporation, the solid phase precludes incorporation of additional RNA while allowing RNA-dependent partitioning of client proteins. Genetic modification of scaffold granule proteins, or tethering the intrinsically disordered region of human Fused in Sarcoma (FUS) to oskar mRNA, allowed modulation of granule material properties in vivo. The resulting liquid-like properties impaired oskar localization and translation with severe consequences on embryonic development. Our study reflects how physiological phase transitions shape RNA-protein condensates to regulate localization and expression of a maternal RNA that instructs germline formation.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.

This dataset comprises the raw data associated with Wong et al., EMBO J., 2024. "Regulation of centrosome size by the cell cycle oscillator in Drosophila embryos". This dataset (approximately 900Gb in total) tracks the behavior of individual centrosomes (labelled with different centrosome markers) in early Drosophila embryos during nuclear cycles 11-13 under various conditions. The datasets are organised to align with each individual Figure shown in the paper. The paper abstract is: Mitotic centrosomes assemble when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. In early C. elegans embryos, mitotic centrosome size appears to be set by the limiting amount of a key component. In Drosophila syncytial embryos, thousands of mitotic centrosomes are assembled as the embryo proceeds through 13 rounds of rapid nuclear division, driven by a core cell-cycle oscillator. These divisions slow during nuclear cycles 11-13, and we find that centrosomes respond by reciprocally decreasing their growth rate, but increasing their growth period—so that they grow to a relatively consistent size at each cycle. At the start of each cycle, moderate CCO activity initially promotes centrosome growth, in part by stimulating Polo/PLK1 recruitment to centrosomes. Later in each cycle, high CCO activity inhibits centrosome growth by suppressing the centrosomal recruitment and/or maintenance of centrosome proteins. Thus, in fly embryos, mitotic centrosome size appears to be regulated predominantly by the core cell-cycle oscillator, rather than by the depletion of a limiting component.