Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
This dataset includes microscopy and sequence data related to the study and used in the figures. It includes light and electron microscopy images. The sample preparation, image acquisition and analysis protocols are described in the methods.
Chromenylium Green, the next generation Indocyanine Green with extended circulatory half-life for high-resolution vascular imaging
BioImage Archive:S-BIAD3372 · (UCLA - University of California, Los Angeles) · Oscheius sp. TEL-2014
Indocyanine Green (ICG) has seen widespread use in the operating room as a fluorescent vascular imaging agent. However, its rapid vascular clearance often necessitates redosing, resulting in procedural delays, greater background, and potential adverse physiological effects. Here, we present Chromenylium Green (ChromG), a novel fluorescent tracer exhibiting excellent visualization of vasculature in mice with greater contrast and seven times the vascular half-life relative to ICG. ChromG allows for high-resolution imaging of vasculature at greater depths, enabling whole-body 3D vascular reconstruction with up to 74% connectivity. As a proof of concept of ChromG’s utility in disease contexts, we demonstrate the ability to visualize saphenous artery stenoses above the clinically accepted contrast limit for eight times longer than ICG. With its excellent biocompatibility profile and compatibility with current clinical imaging technologies, ChromG is a promising fluorescent probe for prolonged, high-resolution intraoperative vascular imaging.
Chromenylium Green, the next generation Indocyanine Green with extended circulatory half-life for high-resolution vascular imaging
BioImage Archive:S-BIAD3372 · (UCLA - University of California, Los Angeles) · Palpoteleia sp. ZL-2020
Indocyanine Green (ICG) has seen widespread use in the operating room as a fluorescent vascular imaging agent. However, its rapid vascular clearance often necessitates redosing, resulting in procedural delays, greater background, and potential adverse physiological effects. Here, we present Chromenylium Green (ChromG), a novel fluorescent tracer exhibiting excellent visualization of vasculature in mice with greater contrast and seven times the vascular half-life relative to ICG. ChromG allows for high-resolution imaging of vasculature at greater depths, enabling whole-body 3D vascular reconstruction with up to 74% connectivity. As a proof of concept of ChromG’s utility in disease contexts, we demonstrate the ability to visualize saphenous artery stenoses above the clinically accepted contrast limit for eight times longer than ICG. With its excellent biocompatibility profile and compatibility with current clinical imaging technologies, ChromG is a promising fluorescent probe for prolonged, high-resolution intraoperative vascular imaging.
Chromenylium Green, the next generation Indocyanine Green with extended circulatory half-life for high-resolution vascular imaging
BioImage Archive:S-BIAD3372 · (UCLA - University of California, Los Angeles) · Pericapritermes sp. Cam20-LS-Eri
Indocyanine Green (ICG) has seen widespread use in the operating room as a fluorescent vascular imaging agent. However, its rapid vascular clearance often necessitates redosing, resulting in procedural delays, greater background, and potential adverse physiological effects. Here, we present Chromenylium Green (ChromG), a novel fluorescent tracer exhibiting excellent visualization of vasculature in mice with greater contrast and seven times the vascular half-life relative to ICG. ChromG allows for high-resolution imaging of vasculature at greater depths, enabling whole-body 3D vascular reconstruction with up to 74% connectivity. As a proof of concept of ChromG’s utility in disease contexts, we demonstrate the ability to visualize saphenous artery stenoses above the clinically accepted contrast limit for eight times longer than ICG. With its excellent biocompatibility profile and compatibility with current clinical imaging technologies, ChromG is a promising fluorescent probe for prolonged, high-resolution intraoperative vascular imaging.
This dataset contains comprehensive image-based morphological profiling data from a systematic drug repurposing screen for host-directed antivirals. The repository includes three major study components.
The primary screen employed high-content Cell Painting analysis combined with viral immunostaining of 5,275 repurposable compounds screened against SARS-CoV-2 infection in Vero E6 monkey kidney cells. This initial morphological profiling identified compounds that reversed the infected cell phenotype towards non-infected controls.
Hit validation was performed through dose-response morphological profiling experiments by Cell Painting and antibody staining in human A549 lung epithelial cells engineered to express the ACE2 receptor, confirming antiviral activity in a human-relevant cell model.
A counter screen assessed compound antiviral activity that could be attributed to drug-induced phospholipidosis (DIPL) instead of specific engagement of viral or host targets. This component includes high-content data from human A549-ACE2 cells stained with HCS LipidTOX Green Phospholipidosis Detection Reagent, CellTracker Deep Red Dye, and Hoechst 33342.
The dataset includes raw images and extracted morphological features. This resource enables identification of host-targeting antivirals with potential broad-spectrum activity and supports future pandemic preparedness efforts.
This dataset includes microscopy and sequence data related to the study and used in the figures. It includes light and electron microscopy images. The sample preparation, image acquisition and analysis protocols are described in the methods.
This dataset includes microscopy and sequence data related to the study and used in the figures. It includes light and electron microscopy images. The sample preparation, image acquisition and analysis protocols are described in the methods.
