Cellular & Molecular Imaging

Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.

curl "https://<hub-domain>/api/v1/cellular-imaging"

Drosophila Alms1 proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification

BioImage Archive:S-BIAD1481 · Veronique Morel (Centre National de la Recherche Scientifique) · Drosophila melanogaster

raw data of manuscript

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publicrestrictedAFDSI-CELL-317

Description of Salileptolyngbya dominicana sp. nov. (Cyanobacteria), through a Diversity Survey of Freshwater, Saline, and Thermal Environments from Dominican Republic

BioImage Archive:S-BIAD2525 · (Interdisciplinary Centre of Marine and Environmental Research) · Dicroscelio sp. ZL-2020

Polyphasic studies on cyanobacterial biodiversity are scarce in the Caribbean and have never been conducted in the Dominican Republic. In this study we analysed sixteen cyanobacterial isolates sampled from biofilms growing on rocks and related to freshwater, saline and thermal environments across the country. A polyphasic approach was employed, incorporating 16S rRNA gene phylogenetic analysis, molecular identity assessments (p-distance), 16S–23S ITS sequence comparison and secondary structure analysis, alongside morphological characterization and habitat comparison. The strains were distributed across the orders Nodosilineales, Oculatellales, Oscillatoriales and Nostocales. Eleven strains were identified as belonging to Almyronema, Euryhalinema, Salileptolyngbya, Nodosilinea, Reticulonema, Vacuolonema, Capilliphycus, Purpureonostoc, Violetonostoc, Desmonostoc and Hapalosiphon. Additionally, four strains formed three distinct clades and presented low 16S rRNA gene identities with their phylogenetically closest genera, suggesting the existence of three undescribed lineages pending comprehensive polyphasic study. At the species level, four strains were considered undescribed lineages pending comprehensive polyphasic study and one strain (LEGE 171504) was described as a new species of Salileptolyngbya. This study represents the first investigation into the cyanobacterial diversity of Dominican Republic expanding the distribution of the identified taxa. Moreover, this is the first report of Salileptolyngbya in the Caribbean region and in brackish waters, as well as the first newly described cyanobacterial species from the Dominican Republic.

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publicrestrictedAFDSI-CELL-233

An integrated anatomical, functional and evolutionary view of the Drosophila olfactory system

BioImage Archive:S-BIAD1758 · Jérôme Mermet (University of Lausanne) · Drosophila melanogaster

The Drosophila melanogaster olfactory system is one of the most intensively studied parts of the nervous system in any animal. Composed of ~50 independent olfactory neuron classes, with several associated hygrosensory and thermosensory pathways, it has been subject to diverse types of experimental analyses. However, synthesizing the available information is limited by the incomplete data and inconsistent nomenclature found in the literature. In this work, we first “complete” the peripheral sensory map through the identification of a previously uncharacterized antennal sensory neuron population expressing Or46aB, and the definition of an exceptional “hybrid” olfactory neuron class comprising functional Or and Ir receptors. Second, we survey developmental, anatomical, connectomic, functional and evolutionary studies to generate an integrated dataset and associated visualisations of these sensory neuron pathways, creating an unprecedented resource. Third, we illustrate the utility of the dataset to reveal relationships between different organizational properties of this sensory system, and the new questions these stimulate. Such examples emphasize the power of this resource to promote further understanding of the construction, function and evolution of these neural circuits.

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publicrestrictedAFDSI-CELL-318

Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera

BioImage Archive:S-BIAD24 · Cercopithecus mona

IF based assay for Covid19 antibodies based on high-throughput microscopy.

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publicrestrictedAFDSI-CELL-827

A large collection of Scanning Electron Microscopy images of protists and their taxonomic annotations from the Marquesas Island area (Tara Oceans survey, Southern Pacific Ocean).

BioImage Archive:S-BIAD598 · Adriana Zingone (Stazione Zoologica Anton Dohrn) · Dicroscelio sp. ZL-2020

Tara Expeditions are global scientific voyages that probe morphological and molecular diversity, evolution and ecology of marine plankton to explore how they are impacted by changes in the Earth's climate. The first expeditions collected samples of marine plankton containing viruses, bacteria, archaea, protists and planktonic metazoans living in the photic layer of the world's oceans. These expeditions, the first taking place between 2009 and 2013, include Tara Oceans: a global view, and Tara Oceans Polar Circle, both of which followed the same sampling protocol. This dataset includes 1074 pictures of 284 planktonic taxa (mainly microalgae and other Ciliate and Radiolarian protists) collected from the vicinity of the Marquesas Islands in the Southern Pacific Ocean during the Tara Oceans expedition. Multiple samples particularly of the size fractions 5-20 and 20 180 um from four sites and two depths were processed with different methods and studied in detail using scanning electron microscopy.

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publicrestrictedAFDSI-CELL-234

BioImage Archive:S-BIAD843 · Giulia Paci · Drosophila melanogaster

Individual 3D cell shapes of Drosophila Wing Disc

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publicrestrictedAFDSI-CELL-319

Rosetta gen. nov. holotype and isotype LM and SEM

BioImage Archive:S-BIAD767 · Casey Engstrom (Simon Fraser University) · Dicroscelio sp. ZL-2020

Light microscopy and scanning electron microscopy images of snow algae field samples containing Rosetta gen. nov., including type material.

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publicrestrictedAFDSI-CELL-235

Supplementary Dataset for "Pore-scale hydrodynamics influence the spatial evolution of bacterial biofilms in a microfluidic porous network"

BioImage Archive:S-BSST244 · Dicroscelio sp. ZL-2020

Bacteria occupy heterogeneous environments, attaching and growing within pores in materials, living hosts, and matrices like soil. Systems that permit high-resolution visualization of dynamic bacterial processes within the physical confines of a realistic and tractable porous media environment are rare. Here we use microfluidics to replicate the grain shape and packing density of natural sands in a 2D platform to study the flow-induced spatial evolution of bacterial biofilms underground. We introduce a wildtype strain (Pantoea sp. YR343, n=3) or an EPS-defective strain (Pantoea sp. YR343 ΔUDP, n=3) to the porous media platform and then simulate a rainfall event using gravity-driven flow of bacterial growth media.

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publicrestrictedAFDSI-CELL-236

Drosophila SOP cells treated with RNAi

BioImage Archive:S-BIAD1473 · Vicente Jose Planelles Herrero (MRC Laboratory of Molecular Biology) · Drosophila melanogaster

Drosophila notum containing SOP cells (labelled with RFP-Pon). Stained for total tubulin and poly-glutamylated tubulin.

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publicrestrictedAFDSI-CELL-320

Calcium Imaging data for Zhu et. al 2024 elife

BioImage Archive:S-BIAD1314 · Tomoko Ohyama (McGill University) · Drosophila melanogaster

This is row data for Figure 4 and 6 data for Zhu et.al 2024 elife.

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publicrestrictedAFDSI-CELL-321

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