Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
Whole-organ single-cell mapping defines growth dynamics and clonal organization in developing and adult zebrafish livers
BioImage Archive:S-BIAD3622 · (Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, 11529, Taiwan) · Diceros bicornis minor
Vertebrate organs undergo massive expansion during post-embryonic growth, yet how this process is organized across entire organs at single-cell resolution remains unclear. This has been limited by the inability to resolve individual cells throughout intact adult organs at sufficient resolution and with cell-type specificity. Here we establish whole adult-organ expansion microscopy (WAO-ExM), enabling in toto single-cell-resolved visualization of every hepatocyte within a complete adult vertebrate liver. Using transgenic reporters to label hepatocyte nuclei, we readily capture 1,265,206 cells within an intact adult liver spanning ~5 mm in thickness and quantify their expansion dynamics across the entire post-embryonic growth period. We reveal a non-linear growth regime in which hepatocyte number increases by 538-fold, driven by a temporally concentrated burst that is not reflected by overall body growth. Lineage tracing integrated with WAO-ExM determines that this expansion is not even, with a few hepatocytes undergoing drastic clonal expansion at the whole-organ scale. Perturbation of extracellular matrix laminins further decouples liver shaping process from total cell number increase, demonstrating independent regulatory control of tissue architecture and cell proliferation. This framework extends to diseased livers and other adult organs, including the heart and pancreas. Altogether, these findings define the cell-level dynamics underlying centimeter-scale organ-level growth, and we demonstrate a generalizable platform for adult vertebrate organ to be fully resolved at bona fide single-cell resolution.
Synthetic protein binders reveal a cryptic regulatory pocket on Aurora A for selective allosteric inhibition
BioImage Archive:S-BIAD2827 · (University of Oxford) · Homo sapiens
"Imaging source data used to create figures in the paper "Synthetic protein binders reveal a cryptic regulatory pocket on Aurora A for selective allosteric inhibition"."
BioImage Archive:S-BIAD584 · Chenghao Guo (Southeast University) · Homo sapiens
The imaging datasets from "SEC mediates phase transition of SPT5 during transcriptional pause release" are publicly available via the BioImage Archive database
