Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
The Darwin Tree of Life project has the goal to sequence the genomes of 70,000 species of eukaryotic organisms in Britain and Ireland. This is a collection of photographs of the samples included in the study, provided by the National History Museum (NHM).
Neoadjuvant PD-L1/TGFβ Blockade Can Rapidly Disrupt the Tumor Stroma in Head and Neck Cancer Patients
BioImage Archive:S-BIAD4011 · (Institut Curie) · Diceros bicornis minor
TGFβ drives fibroblast activation in cancer and is a resistance biomarker to PD-1/PD-L1 blockade.
Focusing on the tumor stroma, we characterized the impact of neoadjuvant treatment with bintrafusp-alfa (BA), a dual PD-L1 and TGFβ inhibitor, in a window-of-opportunity study with six head and neck squamous cell carcinoma (HNSCC) patients. Tumor biopsies pre-treatment and 3-weeks post-therapy were analyzed using single-cell RNA sequencing, imaging and spatial transcriptomics.
After treatment, we observed significant decreased levels of activated LRRC15+ cancer-associated fibroblasts (CAF); remodeled stromal architecture with reduced collagen I and XII content; and increased CD8+ T-cell infiltration in immune-excluded tumors. These changes were independently confirmed in an additional neoadjuvant BA-treated cohort of six HNSCC patients. The drastic CAF and stromal compartment reduction observed indicated a fibroblast depletion rather than a fibroblast polarization shift.
While our final clinical cohort does not allow to assess the treatment clinical effect, this study demonstrated that targeting TGFβ in patients can disrupt the tumor stroma within a short timeframe. Further trials and novel biomarkers are now needed to select patients who could benefit.
The Darwin Tree of Life project has the goal to sequence the genomes of 70,000 species of eukaryotic organisms in Britain and Ireland. This is a collection of photographs of the samples included in the study, provided by the National History Museum (NHM).
The Darwin Tree of Life project has the goal to sequence the genomes of 70,000 species of eukaryotic organisms in Britain and Ireland. This is a collection of photographs of the samples included in the study, provided by the National History Museum (NHM).
Diffusible fraction of niche BMP ligand safeguards stem-cell differentiation
BioImage Archive:S-BIAD1008 · Sharif M. Ridwan (University of Connecticut Health Center) · Drosophila orena
In Drosophila male gonads, niche-derived Decapentaplegic (Dpp) has a role in maintaining stem cells in close proximity but the range of Dpp diffusion and its role has never been tested in the differentiating cells spaced one-cell layer away. Here, using genetically encoded nanobodies called Morphotraps, we physically block Dpp diffusion without interfering with niche-stem cell signaling and find that a diffusible fraction of Dpp is required to ensure differentiation of GSC daughter cells, opposite of its role in maintenance of GSC in the niche. Our work provides an example in which a soluble niche ligand induces opposed cellular responses in stem cells versus differentiating descendants to ensure spatial control of the niche, which may be a common mechanism to regulate tissue homeostasis.
