Cellular & Molecular Imaging

histology

Reprocessed images from LINCS image dataset and Human Protein Atlas for training deep learning models.

raw data of manuscript

Individual 3D cell shapes of Drosophila Wing Disc

Plant plasma membrane proteins are not uniformly distributed in the membrane; however, the principles governing their organization remain not fully understood. HYPERSENSITIVE-INDUCED REACTION (HIR) proteins are plant-specific members of the stomatin/prohibitin/flotillin/HflK/C (SPFH) family implicated in membrane organization. Arabidopsis thaliana HIR2 interacts with multiple plasma membrane proteins, including receptor kinases such as BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE (BAK1)-INTERACTING RECEPTORS 2 and 3 (BIR2 and 3) and BAK1. These interactions connect HIR2 to BAK1-mediated signaling pathways, as evidenced by impaired growth and immunity phenotypes in hir2 mutants. HIR2 is anchored to the inner leaflet of the plasma membrane through a hydrophobic domain and S-acylation. Single-particle-tracking photoactivated localization microscopy (sptPALM) revealed that HIR2 affects BAK1 mobility and clustering exemplifying a role in regulating plasma membrane dynamics. Structural modeling with AlphaFold 3 predicts a multimeric cup-like assembly for HIR2, consistent with high molecular weight complexes identified through blue native PAGE. These findings indicate that HIR2 contributes to the formation of membrane sub-compartments, providing a potential structural framework for spatial membrane organization that influences the dynamics and function of membrane-resident receptors.

Large-area electron microscopy of metabolic tissues from mouse, human and guinea pig — pancreatic islet and pancreatic islet organoid, liver, brown adipose tissue, skeletal muscle, kidney, small intestine and brain — sampled across dietary intervention (high-fat diet, calorie restriction), glucose infusion and ageing. 351 images at mostly 5 nm pixel size: 311 by scanning electron microscopy and 40 by transmission electron microscopy. Files are organised into one folder per dataset. Each folder is named with a short key of the form <species>_<tissue><n>, where ms = Mus musculus, hm = Homo sapiens and gp = Cavia porcellus (for example ms_islet1, hm_liver2). The key table supplied with this submission, Submission_ArrojoeDrigo_internal_EM_key_table.tsv, maps every key to a description of its contents; one dataset (ms_multi4) is further divided into nested subfolders. The file list repeats the key alongside the organism, organ, cell type, imaging method and sample attributes for every image.

The European Reference Genome Atlas (ERGA) initiative is a pan-European scientific response to current threats to biodiversity. Reference genomes provide the most complete insight into the genetic basis that forms each species and represent a powerful resource in understanding how biodiversity functions. This is a collection of the samples included in the study, provided by COPO at Earlham Institute.

Lepidoptera, i.e. butterflies and moths, are vital components of the global ecosystem. Project Psyche is a scientific research project established to sequence the genomes of all butterflies and moths of Europe; helping to conserve, protect and drive innovation.

A library of 1024 pesticides was screened at sublethal doses for effects on the behaviour of Drosophila third instar larvae.

Xenium prime (5K) on healthy human lung samples from parenchyma or bronchi (aiming for first bronchial branch). These samples were obtained from organ donors from the Collaborative Biorepository for Translational Medicine (CBTM) in Cambridge, UK and the majority will be from elderly donors. Note that 10X Flex data is also available for these samples in addition to Xenium uploaded here.