Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
Correlative three-dimensional X-ray histology (3D-XRH) as a tool for quantifying mammalian placental structure
BioImage Archive:S-BIAD1270 · Davis Laundon (University of Southampton) · Bos taurus
Correlative 3D microCT-2D H&E Histology datasets associated with the publication 'Correlative three-dimensional X-ray histology (3D-XRH) as a tool for quantifying mammalian placental structure'
Confocal imaging data for "Volumetric morphometry reveals mitotic spindle width as the best predictor of spindle scaling"
BioImage Archive:S-BSST669 · Bos taurus
Raw confocal imaging data sets of mitotic cells of various cell types with fluorescently-labeled chromatin and tubulin. For our study, we developed an open-source 3D morphometric analysis tool "Spindle3D" (https://sites.imagej.net/Spindle3D) to robustly and reproducibly derive morphometric quantifications from confocal spindle images.
The Darwin Tree of Life project has the goal to sequence the genomes of 70,000 species of eukaryotic organisms in Britain and Ireland. This is a collection of photographs of the samples included in the study, provided by the National History Museum (NHM).
Light My Cells : Bright Field to Fluorescence Imaging Challenge
BioImage Archive:S-BIAD1047 · Dorian Kauffmann (Laboratoire d’informatique, de robotique et de microélectronique de Montpellier, LIRMM, University of Montpellier CNRS, Montpellier, France.) · Babesia canis rossi
We present the Light My Cells Challenge Database, a large-scale open-access collection of 56,981 microscopy images designed to support the development of machine learning models for fluorescence prediction from transmitted light images. Acquired across 30 independent studies from 8 national imaging centers, the dataset captures a wide diversity of biological samples, imaging modalities, and acquisition systems. Each transmitted light image—recorded in bright-field, phase contrast, or differential interference contrast—is paired with at least one fluorescence image labeling key subcellular structures: nucleus, mitochondria, tubulin, or actin. All images are standardized in the OME-TIFF format and annotated with rich metadata following the REMBI guidelines. A dedicated preprocessing pipeline ensures dimensional harmonization, best-focus plane selection, and consistent file naming. The database reflects the variability encountered in real-life microscopy experiments, making it ideally suited for training and benchmarking generalizable deep learning models. It is accessible via the BioImage Archive and supports a range of downstream applications, including in silico labeling, segmentation, and cell profiling from label-free imaging.
Mitochondrial damage drives T-cell immunometabolic paralysis after major surgery
BioImage Archive:S-BIAD2197 · (LMU) · Broadleysaurus major
Cytotoxic T cell (CTL) dysfunction is a key feature of immune paralysis following major surgery, significantly increasing the risk of severe nosocomial infections and contributing to elevated mortality in critically ill patients. The pathomechanisms of CTL dysfunction remain unclear. We report that reactive oxygen species (ROS) release by Myeloid-Derived Suppressor Cells, which transiently and unexpectedly emerge after major surgery, drives perioperative CTL immunoparalysis. This ROS release causes a critical accumulation of ROS within CTL, overwhelming their antioxidative defenses and severely compromising mitochondrial membrane potential. Consequently, oxidative phosphorylation is impaired, and CTL effector functions are inhibited. Additionally, stress-induced mitochondrial hyperfusion occurs, which disrupts fission-dependent mitochondrial translocation to the immunological synapse, exacerbating the bioenergetic failure. These processes result in substantial mitochondrial dysfunction, which could be partially reversed by treatment with the mitochondria-targeting antioxidant MitoTEMPO. Stabilizing mitochondrial function could serve as a promising clinical strategy for preventing and treating perioperative immune dysfunction.
The Darwin Tree of Life project has the goal to sequence the genomes of 70,000 species of eukaryotic organisms in Britain and Ireland. This is a collection of photographs of the samples included in the study, provided by the National History Museum (NHM).
BioImage Archive:S-BIAD588 · Inez Januszczak (Natural History Museum, London) · Bagrus meridionalis
The Darwin Tree of Life project has the goal to sequence the genomes of 70,000 species of eukaryotic organisms in Britain and Ireland. This is a collection of photographs of the samples included in the study, provided by the National History Museum (NHM).
