Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
BioImage Archive:S-BIAD571 · Clara Taffoni (Institute of Human Genetics) · Motacilla clara
Cytosolic DNAs promote inflammatory responses upon detection by the cyclic GMP-AMP (cGAMP) synthase (cGAS). It has been thus suggested that cGAS downregulation is an immune escape strategy harnessed by tumor cells. Here, we used glioblastoma cells that present undetectable cGAS levels to question whether alternative DNA detection pathways can promote pro-inflammatory signaling. We show that the DNA-PK DNA repair complex drives cGAS-independent inflammatory responses but that its catalytic activity is required for cGAS-dependent cGAMP production and optimal downstream signaling. We further show that the cooperation between DNA-PK and cGAS favors the expression of chemokines that promote macrophage recruitment in the tumor microenvironment, a process that impairesd early tumorigenesis but correlatesd with poor outcome. Thus, our study supports that cGAS-dependent signaling is acquired during tumorigenesis and that cGAS and DNA-PK activities should be analyzed concertedly to predict the impact of strategies aiming to boost tumor immunogenicity.
Asymmetric localization of oskar RNP granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP granules in the oocyte are condensates with solid-like physical properties. Using purified oskar RNA and scaffold proteins Bruno and Hrp48, we confirm in vitro that oskar granules undergo a liquid-to-solid phase transition. Whereas the liquid phase allows RNA incorporation, the solid phase precludes incorporation of additional RNA while allowing RNA-dependent partitioning of client proteins. Genetic modification of scaffold granule proteins, or tethering the intrinsically disordered region of human Fused in Sarcoma (FUS) to oskar mRNA, allowed modulation of granule material properties in vivo. The resulting liquid-like properties impaired oskar localization and translation with severe consequences on embryonic development. Our study reflects how physiological phase transitions shape RNA-protein condensates to regulate localization and expression of a maternal RNA that instructs germline formation.
Asymmetric localization of oskar RNP granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP granules in the oocyte are condensates with solid-like physical properties. Using purified oskar RNA and scaffold proteins Bruno and Hrp48, we confirm in vitro that oskar granules undergo a liquid-to-solid phase transition. Whereas the liquid phase allows RNA incorporation, the solid phase precludes incorporation of additional RNA while allowing RNA-dependent partitioning of client proteins. Genetic modification of scaffold granule proteins, or tethering the intrinsically disordered region of human Fused in Sarcoma (FUS) to oskar mRNA, allowed modulation of granule material properties in vivo. The resulting liquid-like properties impaired oskar localization and translation with severe consequences on embryonic development. Our study reflects how physiological phase transitions shape RNA-protein condensates to regulate localization and expression of a maternal RNA that instructs germline formation.
A large collection of Scanning Electron Microscopy images of protists and their taxonomic annotations from the Marquesas Island area (Tara Oceans survey, Southern Pacific Ocean).
Tara Expeditions are global scientific voyages that probe morphological and molecular diversity, evolution and ecology of marine plankton to explore how they are impacted by changes in the Earth's climate. The first expeditions collected samples of marine plankton containing viruses, bacteria, archaea, protists and planktonic metazoans living in the photic layer of the world's oceans. These expeditions, the first taking place between 2009 and 2013, include Tara Oceans: a global view, and Tara Oceans Polar Circle, both of which followed the same sampling protocol.
This dataset includes 1074 pictures of 284 planktonic taxa (mainly microalgae and other Ciliate and Radiolarian protists) collected from the vicinity of the Marquesas Islands in the Southern Pacific Ocean during the Tara Oceans expedition. Multiple samples particularly of the size fractions 5-20 and 20 180 um from four sites and two depths were processed with different methods and studied in detail using scanning electron microscopy.
Data repository for "Curvature gradient drives polarized tissue flow in the Drosophila embryo"
BioImage Archive:S-BIAD602 · Emily Gehrels (Developmental Biology Institute of Marseille) · Drosophila immigrans
Two photon microscopy time series and lightsheet microscopy z-stack and time series of early Drosophila embryogenesis during posterior midgut invagination.
Data repository for "Curvature gradient drives polarized tissue flow in the Drosophila embryo"
BioImage Archive:S-BIAD602 · Emily Gehrels (Developmental Biology Institute of Marseille) · Drosophila simulans
Two photon microscopy time series and lightsheet microscopy z-stack and time series of early Drosophila embryogenesis during posterior midgut invagination.
