Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
The nitrogen nutrition status affects the main factors of rice yield. In traditional rice nitrogen nutrition monitoring methods, most experts enter the farmland to observe leaf color and growth and apply an appropriate amount of nitrogen fertilizer according to the results. However, this method is laborand time-consuming. To realize automatic rice nitrogen nutrition monitoring, we constructed the Jiangxi rice nitrogen nutrition monitoring model based on a convolution neural network (CNN) using the same region rice canopy image in different generation periods. The results show that the same CNN model could distinguish the rice nitrogen nutrition status in different periods, which can completely realize the automatic discrimination of nitrogen nutrition status so as to guide the scientific nitrogen application of rice in this area.
Asymmetric localization of oskar RNP granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP granules in the oocyte are condensates with solid-like physical properties. Using purified oskar RNA and scaffold proteins Bruno and Hrp48, we confirm in vitro that oskar granules undergo a liquid-to-solid phase transition. Whereas the liquid phase allows RNA incorporation, the solid phase precludes incorporation of additional RNA while allowing RNA-dependent partitioning of client proteins. Genetic modification of scaffold granule proteins, or tethering the intrinsically disordered region of human Fused in Sarcoma (FUS) to oskar mRNA, allowed modulation of granule material properties in vivo. The resulting liquid-like properties impaired oskar localization and translation with severe consequences on embryonic development. Our study reflects how physiological phase transitions shape RNA-protein condensates to regulate localization and expression of a maternal RNA that instructs germline formation.
Asymmetric localization of oskar RNP granules to the oocyte posterior is crucial for abdominal patterning and germline formation in the Drosophila embryo. We show that oskar RNP granules in the oocyte are condensates with solid-like physical properties. Using purified oskar RNA and scaffold proteins Bruno and Hrp48, we confirm in vitro that oskar granules undergo a liquid-to-solid phase transition. Whereas the liquid phase allows RNA incorporation, the solid phase precludes incorporation of additional RNA while allowing RNA-dependent partitioning of client proteins. Genetic modification of scaffold granule proteins, or tethering the intrinsically disordered region of human Fused in Sarcoma (FUS) to oskar mRNA, allowed modulation of granule material properties in vivo. The resulting liquid-like properties impaired oskar localization and translation with severe consequences on embryonic development. Our study reflects how physiological phase transitions shape RNA-protein condensates to regulate localization and expression of a maternal RNA that instructs germline formation.
TrypTag genome-wide protein localisation project data. Widefield epifluorescence microscope images of protein subcellular localisation in the unicellular eukaryotic pathogen Trypanosoma brucei by endogenous tagging with mNeonGreen (mNG). This deposition includes the localisations, ontology and microscopy data used to build the TrypTag database. Data can also be browsed at TrypTag.org.
If you use this data resource please cite Billington et al. 2023 Nature Microbiology (doi:10.1038/s41564-022-01295-6). We recommend including this citation in the results or methods if TrypTag was used as part of a discovery process. If directly using TrypTag images, please also indicate in the figure legend or similar which images are from TrypTag. If carrying out a large-scale data analysis, please also cite this BioStudies deposition.
Data can be mined via the cellular localization imaging or cellular component GO term searches at the genome database TriTrypDB.org (part of VEuPathDB). If you do, please also cite the genome database.
You may also find the following papers informative: Dean et al. 2016 Trends in Parasitology (doi:10.1016/j.pt.2016.10.009), which describes the original project aims and workflow. Halliday et al. 2019 Molecular and Biochemical Parasitology (doi:10.1016/j.molbiopara.2018.12.003), which describes the localisation ontology with example images and comparison to Leishmania.
Data repository for "Curvature gradient drives polarized tissue flow in the Drosophila embryo"
BioImage Archive:S-BIAD602 · Emily Gehrels (Developmental Biology Institute of Marseille) · Drosophila mauritiana
Two photon microscopy time series and lightsheet microscopy z-stack and time series of early Drosophila embryogenesis during posterior midgut invagination.
Data repository for "Curvature gradient drives polarized tissue flow in the Drosophila embryo"
BioImage Archive:S-BIAD602 · Emily Gehrels (Developmental Biology Institute of Marseille) · Drosophila teissieri
Two photon microscopy time series and lightsheet microscopy z-stack and time series of early Drosophila embryogenesis during posterior midgut invagination.
