Light/fluorescence microscopy of cells & tissues, and electron/cryo-EM imaging of macromolecular structures — a metadata catalog with a durable link back to the source archive, not a hosted image gallery. These are primary research datasets (often multi-GB to multi-TB), so this platform never downloads or stores the underlying imaging data itself. Datasets already in either source archive are ingested via accession paste/CSV at /mirroring by a Continental Admin (no automated harvesting — neither source archive supports geography-filterable search); a dataset not yet in either archive can instead be submitted directly below by any node operator, for Continental Admin review. Either way, whoever submits an accession or a self-submitted dataset is the one asserting African origin — this platform does not verify it.
The Drosophila melanogaster olfactory system is one of the most intensively studied parts of the nervous system in any animal. Composed of ~50 independent olfactory neuron classes, with several associated hygrosensory and thermosensory pathways, it has been subject to diverse types of experimental analyses. However, synthesizing the available information is limited by the incomplete data and inconsistent nomenclature found in the literature. In this work, we first “complete” the peripheral sensory map through the identification of a previously uncharacterized antennal sensory neuron population expressing Or46aB, and the definition of an exceptional “hybrid” olfactory neuron class comprising functional Or and Ir receptors. Second, we survey developmental, anatomical, connectomic, functional and evolutionary studies to generate an integrated dataset and associated visualisations of these sensory neuron pathways, creating an unprecedented resource. Third, we illustrate the utility of the dataset to reveal relationships between different organizational properties of this sensory system, and the new questions these stimulate. Such examples emphasize the power of this resource to promote further understanding of the construction, function and evolution of these neural circuits.
Using lacZ reporter mouse models, we mapped expression of the HH ligand SHH, HH receptors, and GLI transcription factors in fungiform, circumvallate and foliate taste papillae in early and late postnatal and adult stages. In adults we also studied the soft palate, and the geniculate and trigeminal ganglia, which extend afferent fibers to the anterior tongue.
Micro-CT visualization of a promastigote secretory gel (PSG) and parasite plug in the digestive tract of the sand fly Lutzomyia longipalpis infected with Leishmania mexicana
Sagittal section images showing cross-section of the stomodeal valve at point of greatest opening of 15 sand flies (Lutzomyia longipalpis) at various stages of infection with Leishmania mexicana. The images show the effects of an infection and of a second blood meal on the morphology of the foregut and midgut (especially thoracic midgut) compared to uninfected, control flies. Three flies were imaged by micro-CT scan in each of five groups: T7CON flies were 7 days after a non-infected blood meal. T9INF and T12INF flies were 9 and 12 days, respectively, after an infected blood meal. T6INF+ and T9INF+ flies were 6 and 9 days, respectively after an infected bloodmeal and 1 and 4 days, respectively, after a second, non-infected blood meal. The data are discussed in a paper published on 27th August 2021 in PLoS NTDs, which is accessible through the following link: https://doi.org/10.1371/journal.pntd.0009682
Estimating phenotypic traits with morphometry from 16 specimens of thallose liverworts of biological soil crusts collected in Southern Sweden and Germany
BioImage Archive:S-BIAD824 · Kristian Peters (German Center for Integrative Biodiversity Research) · Eleusine coracana subsp. coracana
A reference dataset containing macroscopic and bright-field microscopic images of 16 specimens of thallose liverworts of biological soil crusts collected in Southern Sweden and Germany
Using lacZ reporter mouse models, we mapped expression of the HH ligand SHH, HH receptors, and GLI transcription factors in fungiform, circumvallate and foliate taste papillae in early and late postnatal and adult stages. In adults we also studied the soft palate, and the geniculate and trigeminal ganglia, which extend afferent fibers to the anterior tongue.
The European Reference Genome Atlas (ERGA) initiative is a pan-European scientific response to current threats to biodiversity. Reference genomes provide the most complete insight into the genetic basis that forms each species and represent a powerful resource in understanding how biodiversity functions. This is a collection of the samples included in the study, provided by COPO at Earlham Institute.
In multicellular organisms, tissue architecture reflects not only dedicated patterning genes but the integrated state of core cellular physiology. Whether the same holds for the communities that bacteria build has been difficult to test at scale. To this end, we developed µPULLI, a high-throughput platform that pairs low-magnification, label-free brightfield timelapse microscopy with computer vision. Using µPULLI, we screened a genome-wide Vibrio cholerae transposon library. Our screen revealed that community architecture is governed as much by core physiology as by dedicated biofilm genes: perturbations to central metabolism, cofactor biosynthesis, and cell-envelope architecture each leave distinctive, pathway-specific fingerprints on how a community develops. These fingerprints arise through both transcriptional and non-transcriptional mechanisms, including cell-surface changes invisible to RNA sequencing. Small molecules drive communities predictably through this phenotype space, and the principle holds across taxonomically diverse pathogens. Emergent community architecture thus encodes the integrated physiological state of its constituent cells, revealing a rich, largely untapped layer of biological information that can be read from simple, low-magnification brightfield videos of growing cultures.
