Baobab Index

Toxoplasma gondii is a globally distributed zoonotic parasite, and dromedary camels are important intermediate hosts in arid and semi-arid regions. However, information on T. gondii exposure in camels is lacking in Mauritania, which harbors one of the largest camel populations in West Africa. This study reports the first multi-regional seroepidemiological survey to estimate T. gondii seroprevalence and identify associated risk factors in Mauritanian dromedaries. Between 2023 and 2024, serum samples were collected from 953 camels across eight climatically distinct regions. Anti-T. gondii IgG antibodies were detected using the Modified Agglutination Test (MAT; cutoff≥1:20). Risk factors investigated included geographical region, sex, age group, and season of sampling, using multivariable logistic regression and a mixed-effects linear probability model accounting for regional clustering. The overall seroprevalence was 15.0% (143/953). Exposure varied markedly across regions, ranging from 0% in the hyper-arid northern regions of Adrar and Tagant to 41.7% in the southern Sahelian region of Guidimakha. This pronounced spatial gradient is consistent with contrasting climatic and ecological conditions, as higher rainfall and humidity in the south are hypothesized to favor environmental oocyst survival compared to the extreme aridity of the north. Geographical region and age were independent predictors of seropositivity. Compared with camels from Nouakchott, those from Guidimakha had higher odds of exposure (aOR = 2.63), whereas camels from Trarza had a markedly lower risk (aOR = 0.09). Camels older than 6 years were more than twice as likely to be seropositive as those aged 3-5 years, whereas sex and season were not associated with seropositivity. These findings indicate that T. gondii exposure is widespread in Mauritanian dromedaries and that ecological conditions may influence exposure patterns. The marked spatial heterogeneity supports targeted surveillance and One Health interventions to reduce the potential zoonotic risk associated with camel-derived food products.

The efficacy and safety of the M72/AS01E tuberculosis (TB) candidate vaccine were evaluated in the phase 2b randomized, placebo-controlled trial NCT01755598. In the trial, participants with Mycobacterium tuberculosis sensitization by a positive interferon-gamma release assay received either M72/AS01E or placebo (randomized 1:1) and were followed for 3 years. In this descriptive study, we genetically characterized M. tuberculosis isolates from participants who developed pulmonary TB during the trial to assess variability among isolates and evaluate the possibility of preferential progression from infection to active TB by certain strains in M72/AS01E and placebo recipients. M. tuberculosis isolates were recovered from sputum samples and underwent DNA extraction and sequencing to assess lineage, antibiotic resistance profile, genetic variation, and genomic clusters. One hundred isolates (37 M72/AS01E, 63 placebo) were genetically characterized from 50 participants who developed microbiologically confirmed active pulmonary TB during the trial (20 M72/AS01E, 30 placebo). Diverse M. tuberculosis lineages were identified in both M72/AS01E and placebo recipients. No multidrug-resistant strains were detected. Genetic sequences corresponding to the antigenic components of M72/AS01E, showed low variation in pepA and high variation in PPE18. However, no apparent preferential distribution of variants was observed in M72/AS01E or placebo recipients. Cluster analyses identified 10 genomic clusters with isolates from >1 participant, illustrating complex transmission dynamics and potential of co-infection by multiple strains within individuals. This descriptive analysis showed no apparent preferential distribution of local M. tuberculosis strains in M72/AS01E or placebo recipients. These results support the continued development of this candidate vaccine.

Okra, an economically important vegetable in tropical and subtropical regions, faces severe yield constraints due to sap-sucking pests, particularly jassids (

early characterization of outbreak cases supports rapid decisions. We conducted real-time analysis during the initial outbreak phase in mid-March 2025 of Sierra Leone's first 44 laboratory-confirmed Mpox cases (10 we summarized surveillance data and sequenced 18 early cases with Oxford Nanopore. Firth-penalized logistic regression was employed to explore risk factors for severe disease. median age was 27 years (interquartile range 22 to 35); 68.2% were male. Most cases reported no recent international travel (95.5%). All cases presented with rash; fever occurred in 72.7%. Six cases (13.6%) met severe criteria; no deaths occurred (0 of 44; 95% confidence interval 0 to 8.0). Household secondary attack rate was 7.3% during the study window and 8.2% after completion of follow-up. Sequencing identified two co-circulating sub-lineages consistent with A.2.2 and B.1.6 and a strong APOBEC3 pattern (142 of 212 guanine-to-adenine in thymine-cytosine versus 70 of 212 cytosine-to-thymine in guanine-adenine; exact binomial p approximately 8.6x10 real-time analysis during the initial outbreak phase showed community transmission, quantified household spread, documented two sub-lineages with an APOBEC3 signature, and generated severity hypotheses that immediately informed surveillance and vaccine prioritization. Findings require validation in larger cohorts.

Febrile illness remains a leading cause of morbidity in sub-Saharan Africa despite substantial reductions in malaria transmission. As malaria positive cases decrease, an increasing number of patients with febrile illnesses test negative for We conducted a nanopore metagenomic next-generation sequencing (mNGS) investigation of 168 archived blood samples collected from febrile patients in Kipsamoite and Kapsisywa sites in Nandi County, a highland region in western Kenya experiencing declining malaria transmission, between 2012 and 2020. The study used exploratory taxonomic profiling to identify microbial DNA/RNA signatures in archived plasma samples and described organisms with known or potential clinical relevance, while classifying detected organisms into common commensal, skin-associated, and environmental taxa. Demographic and clinical data linked to samples were used to fit multivariable logistic regression models to assess associations. Sequencing revealed substantial microbial heterogeneity, including frequent detection of common skin/environmental taxa, opportunistic organisms, ubiquitous viruses, and a smaller number of organisms with established fever-causing potential. The detections are interpreted as molecular evidence of microbial nucleic acid, not as proof of active infection or fever causality. Malaria-positive subjects had a higher mean number of identified pathogens compared to malaria-negative subjects (3.78 vs. 2.14; p = 0.002), despite a baseline microbial landscape dominated by commensal flora, environmental organisms, and ubiquitous viruses. Nanopore mNGS is a viable tool for identifying non-malarial febrile pathogens in western Kenya. Future efforts must combine systematic sampling with field-deployable contamination controls and causal confirmation frameworks to optimize regional antimicrobial stewardship and surveillance.

The spread of early farming communities first reached Britain c. 4000 BCE, ultimately displacing or assimilating local foragers. Two millennia later, another wave of migration, associated with the Bell Beaker culture, again transformed the genetic and socio-cultural landscape of Britain. Southwestern England is rich in prehistoric burial monuments, some dating from the Early Neolithic, many of which later saw reuse during the Bell Beaker and Bronze Age periods. We analysed the genomes of 30 individuals dated between c. 3800 and 1400 BCE from 12 sites in southwestern England and identified a sharp genetic shift between c. 3000 and 2500 BCE. All individuals predating ~ 3100 BCE were genetically similar to European Neolithic farmers, whilst those after ~ 2550 BCE genetically resembled people associated with the Bell Beaker culture in Britain. At three sites, Bronze Age individuals were buried in monuments originally constructed more than a millennium earlier by genetically distinct Neolithic communities. Lacking clear cultural or biological links to the original builders and users of these monuments, this second wave of burial activity may reflect an attempt by incoming or later groups to anchor themselves within established landscapes and invoke the symbolic authority of earlier burial places. This study provides the first systematic regional ancient genomic transect of southwestern Britain across the Neolithic-Bronze Age transition, an archaeologically rich region previously understudied from a population genomic perspective.

Malaria remains a significant public health challenge in Nigeria, especially among children under five years. The recent introduction of WHO-recommended malaria vaccines offers renewed hope for reducing malaria-related morbidity and mortality, but caregivers' willingness to vaccinate their children depends on their awareness, perceptions and practices. Guided by the Health Belief Model (HBM), this study investigated caregivers' willingness to vaccinate their children against malaria and the factors associated with this willingness in the Akoko area of Ondo State, Nigeria. A community-based cross-sectional study was conducted among 498 caregivers of children aged 0-59 months across eight rural and semi-urban communities in Akoko, selected using a multi-stage sampling technique. Data were collected using a structured, pre-tested questionnaire alongside malaria testing using rapid diagnostic test (RDT) kits, and analysed with Microsoft Excel and R software using descriptive and inferential statistics, including multivariable logistic regression. The mean age (± SD) of caregivers was 34.5±11.0 years, and most were female (87.1%). Most caregivers reported appropriate malaria preventive practices, particularly mosquito net ownership, use of insecticide-treated nets, and environmental sanitation; preventive practice was significantly associated with caregiver education (p < 0.05). Malaria prevalence among under-five children was 10.1% and varied significantly with age (p = 0.008). Awareness of the malaria vaccine was high (77.9%), predominantly sourced from the health system, and willingness to vaccinate was high (87.9%). The mean overall HBM score was 48.3±13.9 (out of 60). Willingness to vaccinate was significantly associated with religion, trust in vaccine information and HBM category (p = 0.014, p = 0.002, p < 0.001, respectively). In multivariable analysis, caregivers who did not trust the information they received (AOR = 0.16; 95% CI: 0.05-0.53; p = 0.002) and those whose previous vaccination experiences influenced their decision (AOR = 0.24; 95% CI: 0.09-0.71; p = 0.009) had lower odds of willingness to vaccinate, while a higher HBM score was associated with higher odds of willingness (AOR = 1.04; 95% CI: 1.00-1.08; p = 0.030). Malaria preventive practices and health beliefs were generally favourable in this population, and this may have contributed to the relatively low malaria prevalence observed. Trust in health information and positive health beliefs were the factors most strongly associated with caregivers' willingness to vaccinate their children. Strengthening healthcare-worker engagement, improving vaccine communication, and addressing caregivers' informational gaps are recommended to support effective and equitable malaria vaccine uptake.

A large proportion of individuals with tuberculosis (TB) are asymptomatic. The biological and inflammatory underpinnings of asymptomatic TB are unknown and may differ from symptomatic TB. We characterise blood transcriptomic and proteomic profiles in South African community screening vs. health facility-based triage cohorts. Asymptomatic TB shares core transcriptomic and proteomic features with symptomatic TB, including upregulation of innate, interferon and inflammatory pathways and downregulation of T and B cell pathways. Integration of transcriptomic and proteomic data from asymptomatic TB individuals identifies two distinct sub-clusters characterized by higher or lower bacterial burden, blood IFN-γ responses, BMI, and chest radiographic abnormalities, suggesting different disease severity. We identify a new blood transcriptomic signature of asymptomatic TB. However, diagnostic performance of transcriptomic and proteomic markers is weaker for asymptomatic TB than symptomatic TB, suggesting that policy development for community-based, asymptomatic TB screening should not adopt biomarkers developed for symptomatic TB triage without further optimization.

Systemic lupus erythematosus (SLE) is a complex autoimmune disease with a strong genetic component, and lupus nephritis (LN) represents one of its most common and severe organ-specific manifestations. This review synthesises current evidence on the genetic architecture of SLE and LN, with a particular focus on identifying shared and distinct genetic susceptibility loci and highlighting knowledge and data gaps in highly burdened African populations. Across studies, most risk loci, including